Objective: The aim of the present study was to analyze if alterations of peripheral-type benzodiazepine receptor (PBR) characteristics occurred in platelet membranes of patients affected by primary fibromyalgia (FM). Design and methods: Platelets were obtained from 30 patients with FM. Evaluation of kinetic parameters of PBR was performed using [H-3] PK11195 as specific radioligand compared with 16 healthy volunteers. Results: The results showed a significant increase of PBR binding sites value in platelet membranes from FM patients (B-max was 5366 +/- 188 fmol/mg vs. controls, 4193 +/- 341 fmoUmg, mean +/- SEM) (**p < 0.01) but not for affinity value (K-d was 4.90 +/- 0.39 nM vs. controls, 4.74 +/- 0.39 nM, mean +/- SEM) (p > 0.05). Symptom severity scores (pain and tiredness) were positively correlated with B-max. Conclusions: Our results showed an up-regulation of PBR in platelets of FM patients, and this seems to be related to the severity of fibromyalgic symptoms. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.
Peripheral benzodiazepine receptors on platelets of fibromyalgic patients
GIANNACCINI, GINO;BETTI, LAURA;GIUSTI, LAURA;LUCACCHINI, ANTONIO;BOMBARDIERI, STEFANO
2006-01-01
Abstract
Objective: The aim of the present study was to analyze if alterations of peripheral-type benzodiazepine receptor (PBR) characteristics occurred in platelet membranes of patients affected by primary fibromyalgia (FM). Design and methods: Platelets were obtained from 30 patients with FM. Evaluation of kinetic parameters of PBR was performed using [H-3] PK11195 as specific radioligand compared with 16 healthy volunteers. Results: The results showed a significant increase of PBR binding sites value in platelet membranes from FM patients (B-max was 5366 +/- 188 fmol/mg vs. controls, 4193 +/- 341 fmoUmg, mean +/- SEM) (**p < 0.01) but not for affinity value (K-d was 4.90 +/- 0.39 nM vs. controls, 4.74 +/- 0.39 nM, mean +/- SEM) (p > 0.05). Symptom severity scores (pain and tiredness) were positively correlated with B-max. Conclusions: Our results showed an up-regulation of PBR in platelets of FM patients, and this seems to be related to the severity of fibromyalgic symptoms. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
