Objectives: The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared. Design and methods: 2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody. Results and conclusions: We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
|Autori:||GIANNACCINI G; GIUSTI L; SANTINI F; MARSILI A; BETTI L; MASCIA G; PELOSINI C; BARONI S; CIREGIA F; FABBRINI L; LUCACCHINI A; VITTI P; PINCHERA A|
|Titolo:||Tubby protein in human lymphocytes from normal weight and obese subjects|
|Anno del prodotto:||2007|
|Digital Object Identifier (DOI):||10.1016/j.clinbiochem.2007.03.020|
|Appare nelle tipologie:||1.1 Articolo in rivista|