In this study, we investigated the effect of 1,25(OH)(2)D-3 on proteinuria and on the alteration of slit diaphragm-associated proteins induced by anti-Thy 1.1 in Wistar rats. Four groups of animals were studied: group I, anti-Thy 1.1 treated rats; group II, anti-Thy1.1 treated group that at day 2, after the onset of overt proteinuria, started the treatment with 1,25(OH)(2)D-3; group III, normal control rats injected with vehicle alone; group IV, rats that received only 1,25(OH)(2)D-3. At day 2, in group I and II, before the administration of 1,25(OH)(2)D-3 protein excretion was significantly increased when compared to controls. Overt proteinuria was maintained until day 14 in group I whereas in group II protein excretion was significantly reduced from day 3 to day 14. Moreover, treatment with 1,25(OH)(2)D-3 abrogated podocytes injury, detected as desmin expression and loss of nephrin and zonula occludens-1 (ZO-1), two slit diaphragm-associated proteins, and glomerular polyanion staining, that were observed in group I. In conclusion, these results suggest that 1,25(OH)(2)D-3 administrated with a therapeutic regiment may revert proteinuria, counteracting glomerular podocyte injury.
|Autori:||MIGLIORI M; GIOVANNINI L; PANICHI V; FILIPPI C; TACCOLA D; ORIGLIA N; MANNARI; C; CAMUSSI G|
|Titolo:||Treatment with 1,25-dihydroxyvitamin D3 preserves glomerular slit diaphragm-associated protein expression in experimental glomerulonephritis|
|Anno del prodotto:||2005|
|Appare nelle tipologie:||1.1 Articolo in rivista|