The identification of the coccidian species isolated from a sheep flock of Zerasca breed located in the province of Massa (Zeri-Tuscany-Italy) represented the aim of the present study. Individual faecal samples were collected from both young and adult animals and analysed qualiquantitatively by flotation test and with a modified McMaster method, by using a low specific gravity solution (s.g.: 1200), and microscopically examined for the search of coccidian oocysts. Faecal samples resulted positive were dissolved in a 2% K2Cr2O7 solution and maintained in the dark and at the temperature of 22±1° C to allow sporulation of the oocysts. Oocysts were daily checked for sporulation for 3-5 days. Quantitatively, young animals showed a larger number of OPG than adults. Two different species were found in the flock: Eimeria bakuensis (E.ovina) and E. parva. Both species were previously reported on the Italian territory. Among sheep coccidian species, E. bakuensis is considered one of the more pathogenic; for this reason it is possible that, in the flock examined in this study, it is responsible for symptomatic infections and reduction of growth-rate in lambs. In addition to supply useful information on the pathogenicity of isolated species, the identification of coccidian species present in a flock or in a specific geographic area could represent an important epidemiological tool for monitoring and mapping the species present in Tuscany and in the Italian territory.
Isolamento di Eimeria Bakuensis e di Eimeria Parva in un allevamento di pecora Zerasca
PERRUCCI, STEFANIA;BENVENUTI, MARIA NOVELLA;GIULIOTTI, LORELLA
2006-01-01
Abstract
The identification of the coccidian species isolated from a sheep flock of Zerasca breed located in the province of Massa (Zeri-Tuscany-Italy) represented the aim of the present study. Individual faecal samples were collected from both young and adult animals and analysed qualiquantitatively by flotation test and with a modified McMaster method, by using a low specific gravity solution (s.g.: 1200), and microscopically examined for the search of coccidian oocysts. Faecal samples resulted positive were dissolved in a 2% K2Cr2O7 solution and maintained in the dark and at the temperature of 22±1° C to allow sporulation of the oocysts. Oocysts were daily checked for sporulation for 3-5 days. Quantitatively, young animals showed a larger number of OPG than adults. Two different species were found in the flock: Eimeria bakuensis (E.ovina) and E. parva. Both species were previously reported on the Italian territory. Among sheep coccidian species, E. bakuensis is considered one of the more pathogenic; for this reason it is possible that, in the flock examined in this study, it is responsible for symptomatic infections and reduction of growth-rate in lambs. In addition to supply useful information on the pathogenicity of isolated species, the identification of coccidian species present in a flock or in a specific geographic area could represent an important epidemiological tool for monitoring and mapping the species present in Tuscany and in the Italian territory.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
