Psoralea bituminosa L. (= Bituminaria bituminosa L., Fabaceae) is a wild perennial herb widespread in the Mediterranean area. Pterocarpans and flavonoid were isolated and identified from B. bituminosa aerial parts in a previous work (Pistelli 2003). Antioxidant properties of plant extracts and pure compounds were reported in the literature (Rosa 2005). The aim of the present work was to establish P. bituminosa in vitro cultures for the production of large amounts of starting plant material useful as source of active ingredients for pharmaceutical purposes. Suitable protocols for the development of friable calli were studied and the establishment of cultures with low environmental impact and without human health risk were evaluated. Growth parameters and preliminary antioxidant total capacity gave the essential information for the management of the cultures. P. bituminosa extracts from adult plant were analysed by HPLC-DAD-MS in order to quantify their characteristic components (erybraedin C, bitucarpin A and plicatin B). The same procedure will be performed on the in vitro plant material to verify the secondary metabolite production
Aseptic cultures of Psoralea bituminosa L. for improving bioactive constituent production
PISTELLI, LAURA;BERTOLI, ALESSANDRA;BEDINI, LAURA;LUCCHESINI, MARIELLA;PISTELLI, LUISA
2007-01-01
Abstract
Psoralea bituminosa L. (= Bituminaria bituminosa L., Fabaceae) is a wild perennial herb widespread in the Mediterranean area. Pterocarpans and flavonoid were isolated and identified from B. bituminosa aerial parts in a previous work (Pistelli 2003). Antioxidant properties of plant extracts and pure compounds were reported in the literature (Rosa 2005). The aim of the present work was to establish P. bituminosa in vitro cultures for the production of large amounts of starting plant material useful as source of active ingredients for pharmaceutical purposes. Suitable protocols for the development of friable calli were studied and the establishment of cultures with low environmental impact and without human health risk were evaluated. Growth parameters and preliminary antioxidant total capacity gave the essential information for the management of the cultures. P. bituminosa extracts from adult plant were analysed by HPLC-DAD-MS in order to quantify their characteristic components (erybraedin C, bitucarpin A and plicatin B). The same procedure will be performed on the in vitro plant material to verify the secondary metabolite productionI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
