A functional ORF-A is essential for efficient feline immunodeficiency virus replication in lymphocytes We have characterized a series of mutants of the Petaluma strain, derived from p34TF10 and having different combinations of stop codons and increasingly long deletions in ORF-A. Six clones proved fully replicative in fibroblastoid Crandell feline kidney cells and monocyte-derived macrophage cultures but failed to replicate in T cell lines and primary lymphoblasts Cats inoculated with three selected mutants had considerably milder infections than controls given intact ORF-A virus In vivo, the mutants maintained growth properties similar to those in vitro for at least 7 months, except that replication in lymphoid cells was strongly reduced but not ablated. One mutant underwent extensive ORF-A changes without, however, reverting to wild-type Antiviral immune responses were feeble in all cats, suggesting that viral loads were too low to represent a sufficiently powerful antigenic stimulus. (C) 2002 Elsevier Science (USA).
Development of feline immunodeficiency virus ORF-A (tat) mutants: In vitro and in vivo characterization
PISTELLO, MAUROWriting – Original Draft Preparation
;FREER, GIULIAWriting – Original Draft Preparation
;BENDINELLI, MAURO
Writing – Review & Editing
2002-01-01
Abstract
A functional ORF-A is essential for efficient feline immunodeficiency virus replication in lymphocytes We have characterized a series of mutants of the Petaluma strain, derived from p34TF10 and having different combinations of stop codons and increasingly long deletions in ORF-A. Six clones proved fully replicative in fibroblastoid Crandell feline kidney cells and monocyte-derived macrophage cultures but failed to replicate in T cell lines and primary lymphoblasts Cats inoculated with three selected mutants had considerably milder infections than controls given intact ORF-A virus In vivo, the mutants maintained growth properties similar to those in vitro for at least 7 months, except that replication in lymphoid cells was strongly reduced but not ablated. One mutant underwent extensive ORF-A changes without, however, reverting to wild-type Antiviral immune responses were feeble in all cats, suggesting that viral loads were too low to represent a sufficiently powerful antigenic stimulus. (C) 2002 Elsevier Science (USA).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.