A functional ORF-A is essential for efficient feline immunodeficiency virus replication in lymphocytes We have characterized a series of mutants of the Petaluma strain, derived from p34TF10 and having different combinations of stop codons and increasingly long deletions in ORF-A. Six clones proved fully replicative in fibroblastoid Crandell feline kidney cells and monocyte-derived macrophage cultures but failed to replicate in T cell lines and primary lymphoblasts Cats inoculated with three selected mutants had considerably milder infections than controls given intact ORF-A virus In vivo, the mutants maintained growth properties similar to those in vitro for at least 7 months, except that replication in lymphoid cells was strongly reduced but not ablated. One mutant underwent extensive ORF-A changes without, however, reverting to wild-type Antiviral immune responses were feeble in all cats, suggesting that viral loads were too low to represent a sufficiently powerful antigenic stimulus. (C) 2002 Elsevier Science (USA).
|Autori:||Pistello M; Moscardini M; Mazzetti P; Bonci F; Zaccaro L; Isola P; Freer G; Specter S; Matteucci D; Bendinelli M|
|Titolo:||Development of feline immunodeficiency virus ORF-A (tat) mutants: In vitro and in vivo characterization|
|Anno del prodotto:||2002|
|Digital Object Identifier (DOI):||10.1006/viro.2002.1442|
|Appare nelle tipologie:||1.1 Articolo in rivista|