A functional ORF-A is essential for efficient feline immunodeficiency virus replication in lymphocytes We have characterized a series of mutants of the Petaluma strain, derived from p34TF10 and having different combinations of stop codons and increasingly long deletions in ORF-A. Six clones proved fully replicative in fibroblastoid Crandell feline kidney cells and monocyte-derived macrophage cultures but failed to replicate in T cell lines and primary lymphoblasts Cats inoculated with three selected mutants had considerably milder infections than controls given intact ORF-A virus In vivo, the mutants maintained growth properties similar to those in vitro for at least 7 months, except that replication in lymphoid cells was strongly reduced but not ablated. One mutant underwent extensive ORF-A changes without, however, reverting to wild-type Antiviral immune responses were feeble in all cats, suggesting that viral loads were too low to represent a sufficiently powerful antigenic stimulus. (C) 2002 Elsevier Science (USA).

Development of feline immunodeficiency virus ORF-A (tat) mutants: In vitro and in vivo characterization

PISTELLO, MAURO
Writing – Original Draft Preparation
;
FREER, GIULIA
Writing – Original Draft Preparation
;
BENDINELLI, MAURO
Writing – Review & Editing
2002

Abstract

A functional ORF-A is essential for efficient feline immunodeficiency virus replication in lymphocytes We have characterized a series of mutants of the Petaluma strain, derived from p34TF10 and having different combinations of stop codons and increasingly long deletions in ORF-A. Six clones proved fully replicative in fibroblastoid Crandell feline kidney cells and monocyte-derived macrophage cultures but failed to replicate in T cell lines and primary lymphoblasts Cats inoculated with three selected mutants had considerably milder infections than controls given intact ORF-A virus In vivo, the mutants maintained growth properties similar to those in vitro for at least 7 months, except that replication in lymphoid cells was strongly reduced but not ablated. One mutant underwent extensive ORF-A changes without, however, reverting to wild-type Antiviral immune responses were feeble in all cats, suggesting that viral loads were too low to represent a sufficiently powerful antigenic stimulus. (C) 2002 Elsevier Science (USA).
Pistello, Mauro; Moscardini, M; Mazzetti, P; Bonci, F; Zaccaro, L; Isola, P; Freer, Giulia; Specter, S; Matteucci, D; Bendinelli, Mauro
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/188220
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