Tissues from 34 naturally feline immunodeficiency virus (FIV)-infected cats, 13 asymptomatic and 21 with signs of feline acquired immunodeficiency syndrome (F-AIDS), and 35 FIV-seronegative subjects were examined to determine the presence of amyloid deposits. Twenty experimentally FIV-infected cats and five specific pathogen free (SPF) control cats were also included in the study. Paraffin-embedded sections from kidney and other organs were submitted to histological and histochemical analysis and amyloid deposits were identified by a modified Congo Red stain and confirmed by electron microscopy to demonstrate the presence of amyloid fibrils, in amyloid positive glomeruli. In all positive cases, secondary amyloidosis was identified with the potassium permanganate pretreatment and amyloid type was further characterized by immunoistochemistry using primary antibodies against human AA and AL amyloids. Amyloid deposits were present in different tissues of 12/34 (35%) naturally FIV-infected cats (7 presenting FAIDS and 5 in asymptomatic phase) and in 1/30 FIV-seronegative cats. All the experimentally FIV-infected and SPF subjects showed no amyloid deposits. Amyloidosis has been yet reported in lentiviral infections, and the data here reported demonstrate the need, in naturally FIV-infected cats, to consider the presence of amyloidosis in differential diagnosis of hepatic and renal disorders to better assess the prognosis of the disease.

Amyloidosis in association with spontaneous Feline Immunodeficiency Virus infection

ABRAMO, FRANCESCA;MILLANTA, FRANCESCA;POLI, ALESSANDRO
2013

Abstract

Tissues from 34 naturally feline immunodeficiency virus (FIV)-infected cats, 13 asymptomatic and 21 with signs of feline acquired immunodeficiency syndrome (F-AIDS), and 35 FIV-seronegative subjects were examined to determine the presence of amyloid deposits. Twenty experimentally FIV-infected cats and five specific pathogen free (SPF) control cats were also included in the study. Paraffin-embedded sections from kidney and other organs were submitted to histological and histochemical analysis and amyloid deposits were identified by a modified Congo Red stain and confirmed by electron microscopy to demonstrate the presence of amyloid fibrils, in amyloid positive glomeruli. In all positive cases, secondary amyloidosis was identified with the potassium permanganate pretreatment and amyloid type was further characterized by immunoistochemistry using primary antibodies against human AA and AL amyloids. Amyloid deposits were present in different tissues of 12/34 (35%) naturally FIV-infected cats (7 presenting FAIDS and 5 in asymptomatic phase) and in 1/30 FIV-seronegative cats. All the experimentally FIV-infected and SPF subjects showed no amyloid deposits. Amyloidosis has been yet reported in lentiviral infections, and the data here reported demonstrate the need, in naturally FIV-infected cats, to consider the presence of amyloidosis in differential diagnosis of hepatic and renal disorders to better assess the prognosis of the disease.
Asproni, P; Abramo, Francesca; Millanta, Francesca; Lorenzi, D; Poli, Alessandro
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/192525
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