The controversial and limited data on the distribution of dopamine (DA) receptors of type 4 (D(4)) in the human brain prompted us to explore their density and pharmacological characteristics in the prefrontal cortex, striatum and hippocampus, through a series of binding assays. Brain samples were taken during autopsy from seven subjects. Tissue homogenates were incubated with increasing concentration of [(3)H]-YM-09151-2, a D(2)-like receptor antagonist, and L-745,870 and/or sulpiride to define the non-specific binding, while PPAP was used to block sigma receptors. The results showed a low density of D(4) receptors in the hippocampus only, with a preponderance of D(2)/D(3) and sigma receptors in the prefrontal cortex and striatum. In conclusion, these findings underline that it is possible to label D(4) receptors by means of [(3)H]-YM-09151-2, provided that D(2), D(3) and sigma receptors are blocked.
|Autori:||Marazziti D; Baroni S; Masala I; Giannaccini G; Betti L; Palego L; Dell'Osso MC; Consoli G; Castagna M; Lucacchini A|
|Titolo:||[3H]-YM-09151-2 binding sites in human brain postmortem|
|Anno del prodotto:||2009|
|Digital Object Identifier (DOI):||10.1016/j.neuint.2009.06.005|
|Appare nelle tipologie:||1.1 Articolo in rivista|