Sialyloligosaccharides (oligosaccharides containing one or more residues of sialic acid) are relevant components of human milk, which have been quite well studied for their prebiotic effect and their capacity in stimulating the immune system. Since oligosaccharides from milk of non-human mammals received so far less attention, the aim of this work was the application of capillary electrophoresis (CE) for the analysis of sialyloligosaccharides in milk of different species, to possibly identify potential sources of oligosaccharides to use as health promoting ingredients in functional foods. Mare milk (3 samples), ass milk (3 samples), goat milk (2 samples) and cow milk (10 samples), at different stages of lactation, were analysed in comparison to 2 samples of human milk. Samples were frozen at -20°C immediately after collection, thawed over-night at 4°C before analysis, defatted and deproteinized. A recent CE technique using the MEKC mode with direct detection at 200 nm was applied to resolve and quantify 3 of the most dominant sialyloligosaccharides of human milk. Commercial standards of 3-sialyllactose (3-SL), 6-sialyllactose (6-SL) and disialyllacto-N-tetraose (DSLNT) from 39 to 2500 μg/mL were used to create linear calibration curves (R2 from 0.9967 to 0.9997), with coefficients of variation from 3 to 10%. Analysis of non-human milk samples confirmed differences among species, individuals and between stages of lactation: DSLNT, which was the most abundant compound in human milk (455-805 μg/mL) was missing in most of the samples. In most cases 3-SL showed to be the most concentrated of the analytes we quantified, with values ranging from 12 to 77 μg/mL
Capillary electrophoresis analysis of sialyloligosaccharides in milk of different species
CURADI, MARIA CLAUDIA;ORLANDI, MARIO;
2011-01-01
Abstract
Sialyloligosaccharides (oligosaccharides containing one or more residues of sialic acid) are relevant components of human milk, which have been quite well studied for their prebiotic effect and their capacity in stimulating the immune system. Since oligosaccharides from milk of non-human mammals received so far less attention, the aim of this work was the application of capillary electrophoresis (CE) for the analysis of sialyloligosaccharides in milk of different species, to possibly identify potential sources of oligosaccharides to use as health promoting ingredients in functional foods. Mare milk (3 samples), ass milk (3 samples), goat milk (2 samples) and cow milk (10 samples), at different stages of lactation, were analysed in comparison to 2 samples of human milk. Samples were frozen at -20°C immediately after collection, thawed over-night at 4°C before analysis, defatted and deproteinized. A recent CE technique using the MEKC mode with direct detection at 200 nm was applied to resolve and quantify 3 of the most dominant sialyloligosaccharides of human milk. Commercial standards of 3-sialyllactose (3-SL), 6-sialyllactose (6-SL) and disialyllacto-N-tetraose (DSLNT) from 39 to 2500 μg/mL were used to create linear calibration curves (R2 from 0.9967 to 0.9997), with coefficients of variation from 3 to 10%. Analysis of non-human milk samples confirmed differences among species, individuals and between stages of lactation: DSLNT, which was the most abundant compound in human milk (455-805 μg/mL) was missing in most of the samples. In most cases 3-SL showed to be the most concentrated of the analytes we quantified, with values ranging from 12 to 77 μg/mLI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
