In this study, we determined the allele and genotype frequencies of vascular endothelial growth factor (VEGF) G+405C, C–460T, C+936T and C–2578A single nucleotide polymorphisms (SNPs) in 32 patients affected by mantle cell lymphoma (MCL) and 58 healthy controls. Real-time PCR combined with melting curve analysis was used for the determination of SNP alleles. A significant difference in the allele frequency of VEGFC–460T and C+936T SNPs in MCL and healthy cases was not observed. On the contrary, VEGF G+405C and C–2578A SNP allele distribution was significantly lower in the patient group than among normal controls (p = 0.014, p = 0.001). This observation suggests that further investigation is warranted, both in vitro and in a larger series of patients, to further examine the role of VEGF polymorphisms in the pathogenesis of MCL. In addition, the use of quantitative real-time PCR combined with a melting curve analysis method in the detection of the 4 VEGF SNPs may have the potential to replace older and more time-consuming PCR-RFLP methods and bears further investigation. Copyright © 2009 S. Karger AG, Basel

Vascular Endothelial Growth Factor Polymorphisms in Mantle Cell Lymphoma

GALIMBERTI, SARA;BUDA, GABRIELE;RONCA, FRANCESCA;PETRINI, MARIO
2010

Abstract

In this study, we determined the allele and genotype frequencies of vascular endothelial growth factor (VEGF) G+405C, C–460T, C+936T and C–2578A single nucleotide polymorphisms (SNPs) in 32 patients affected by mantle cell lymphoma (MCL) and 58 healthy controls. Real-time PCR combined with melting curve analysis was used for the determination of SNP alleles. A significant difference in the allele frequency of VEGFC–460T and C+936T SNPs in MCL and healthy cases was not observed. On the contrary, VEGF G+405C and C–2578A SNP allele distribution was significantly lower in the patient group than among normal controls (p = 0.014, p = 0.001). This observation suggests that further investigation is warranted, both in vitro and in a larger series of patients, to further examine the role of VEGF polymorphisms in the pathogenesis of MCL. In addition, the use of quantitative real-time PCR combined with a melting curve analysis method in the detection of the 4 VEGF SNPs may have the potential to replace older and more time-consuming PCR-RFLP methods and bears further investigation. Copyright © 2009 S. Karger AG, Basel
Galimberti, Sara; Nagy, B; Palumbo, Ga; Ciancia, E; Buda, Gabriele; Orciuolo, E; Melosi, A; Lambelet, P; Ronca, Francesca; Petrini, Mario
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/195226
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