INACTIVATION OF [S-35] TERT-BUTYLBICYCLOPHOSPHOROTHIONATE BINDING-SITES BY THE ARGININE REAGENT, 2,3-BUTANEDIONE

Martini, Claudia; Pacini, R; Lucacchini, Antonio

doi:10.1016/0197-0186(91)90035-C

The "cage" convulsant [S-35]t-butylbicyclophosphorothionate(S-35]TBPS) binds with high affinity to specific sites "at" or "near" a gamma-amino-butyric acid (GABA)-gated chloride channel according to current hypothesis. We now report that pretreatment of membranes with 2,3-butanedione, an arginine-specific reagent, causes a dose- and time-dependent decrease in the number of [S-35]TBPS binding sites. No decrease occurs when membranes are pretreated with 2,3-butanedione in the presence of picrotoxinin. Binding of [S-35]TBPS to the remaining sites occurs with the same characteristics as binding to the untreated receptor population.

Autori interni:	MARTINI, CLAUDIA PACINI R LUCACCHINI, ANTONIO mostra contributor esterni nascondi contributor esterni
Autori:	MARTINI C; PACINI R; LUCACCHINI A
Titolo:	INACTIVATION OF [S-35] TERT-BUTYLBICYCLOPHOSPHOROTHIONATE BINDING-SITES BY THE ARGININE REAGENT, 2,3-BUTANEDIONE
Anno del prodotto:	1991
Digital Object Identifier (DOI):	10.1016/0197-0186(91)90035-C
Appare nelle tipologie:	1.1 Articolo in rivista

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Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/11568/19543

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