The "cage" convulsant [S-35]t-butylbicyclophosphorothionate(S-35]TBPS) binds with high affinity to specific sites "at" or "near" a gamma-amino-butyric acid (GABA)-gated chloride channel according to current hypothesis. We now report that pretreatment of membranes with 2,3-butanedione, an arginine-specific reagent, causes a dose- and time-dependent decrease in the number of [S-35]TBPS binding sites. No decrease occurs when membranes are pretreated with 2,3-butanedione in the presence of picrotoxinin. Binding of [S-35]TBPS to the remaining sites occurs with the same characteristics as binding to the untreated receptor population.
Autori interni: | |
Autori: | MARTINI C; PACINI R; LUCACCHINI A |
Titolo: | INACTIVATION OF [S-35] TERT-BUTYLBICYCLOPHOSPHOROTHIONATE BINDING-SITES BY THE ARGININE REAGENT, 2,3-BUTANEDIONE |
Anno del prodotto: | 1991 |
Digital Object Identifier (DOI): | 10.1016/0197-0186(91)90035-C |
Appare nelle tipologie: | 1.1 Articolo in rivista |