Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy. EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in a-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to a-MEM. a-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of a-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol. Both a-MEM and MSCBM can be used to cultivate EUCMCs. a-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since a-MEM is more cost-effective than MSCBM.
|Autori interni:||MIRAGLIOTTA, VINCENZO|
STORNELLI, MARIA RITA
|Autori:||Passeri S; Nocchi F; Lamanna R; Lapi S; Miragliotta V; Giannessi E; Abramo F; Stornelli MR; Matarazzo M; Plenteda D; Urciuoli P; Scatena F; Coli A|
|Titolo:||Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs)|
|Anno del prodotto:||2009|
|Digital Object Identifier (DOI):||10.1016/j.cellbi.2008.10.012|
|Appare nelle tipologie:||1.1 Articolo in rivista|