Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy. EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in a-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to a-MEM. a-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of a-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol. Both a-MEM and MSCBM can be used to cultivate EUCMCs. a-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since a-MEM is more cost-effective than MSCBM.
Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs)
MIRAGLIOTTA, VINCENZO;GIANNESSI, ELISABETTA;ABRAMO, FRANCESCA;STORNELLI, MARIA RITA;COLI, ALESSANDRA
2009-01-01
Abstract
Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy. EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in a-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to a-MEM. a-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of a-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol. Both a-MEM and MSCBM can be used to cultivate EUCMCs. a-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since a-MEM is more cost-effective than MSCBM.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
