We analyzed interleukin (IL) 12 and IL-23 production by monocyte-derived dendritic cells (mono-DCs). Mycobacterium tuberculosis H37Rv and zymosan preferentially induced IL-23. IL-23 but not IL-12 was efficiently induced by the combination of nucleotide-binding oligodimerization domain and Toll-like receptor (TLR) 2 ligands, which mimics activation by M. tuberculosis, or by the human dectin-1 ligand beta-glucan alone or in combination with TLR2 ligands, mimicking induction by zymosan. TLR2 ligands inhibited IL-12 and increased IL-23 production. DC priming with interferon (IFN) gamma strongly increased IL-12 production, but was not required for IL-23 production and inhibited IL-23 production induced by beta-glucan. The pattern of IL-12 and IL-23 induction was reflected in accumulation of the IL-12p35 and IL-23p19 transcripts, respectively, but not IL-12/23p40. Although IL-23, transforming growth factor beta, and IL-6 contained in the supernatants of activated mono-DCs played a role in the induction of IL-17 by human CD4(+) T cells, IL-1beta, in combination with one or more of those factors, was required for IL-17 production, and its production determined the differential ability of the stimuli used to elicit mono-DCs to produce soluble factors directing IL-17 production. Thus, the differential ability of pathogens to induce antigen-presenting cells to produce cytokines regulates the immune response to infection.
|Autori:||GEROSA F; BALDANI-GUERRA B; LYAKH LA; BATONI G; ESIN S; WINKLER-PICKETT RT; CONSOLARO MR; DE MARCHI M; GIACHINO D; ROBBIANO A; ASTEGIANO M; SAMBATARO A; KASTELEIN RA; CARRA G; TRINCHIERI G|
|Titolo:||Differential regulation of interleukin 12 and interleukin 23 production in human dendritic cells|
|Anno del prodotto:||2008|
|Digital Object Identifier (DOI):||10.1084/jem.20071450|
|Appare nelle tipologie:||1.1 Articolo in rivista|