Granulocyte function may be altered after in vivo G-CSF administration and this has been related to both an immaturity of mobilized cells and to a defect in F-actin polymerization. In this paper we show that in resting Filgrastim (non-glycosylated G-CSF)-pulsed cells, F-actin polymerization, membrane-linked RhoA and cell polarization are enhanced compared to those found in resting Lenograstim (glycosylated G-CSF)-cells. The basal hyper-activation of RhoA could be responsible for the morphological and functional modifications of Filgrastim-mobilized cells. Moreover, Filgrastim-mobilized cells, but not Lenograstim-mobilized cells, are unable to correctly respond to LPS stimulation, as demonstrated by minor further RhoA activation and cell elongation.
|Autori:||MATTII L; AZZARA' A; FAZZI R; CARULLI G; CHIMENTI M; CECCONI N; GALIMBERTI S; PETRINI M|
|Titolo:||Glycosylated or non-glycosylated G-CSF differently influence human granulocyte functions through RhoA|
|Anno del prodotto:||2005|
|Digital Object Identifier (DOI):||10.1016/j.leukres.2005.04.011|
|Appare nelle tipologie:||1.1 Articolo in rivista|