The purpose of the work was to compare the effects of Kinetin (Kin), N 6-benzylaminopurine (BA) and Thidiazuron (TDZ) on somatic embryo, adventitious root and shoot regeneration from in vitro-cultured quince (Cydonia oblonga Mill.) leaves collected from the apical portion of actively proliferating shoots. After a two-day treatment with 2,4-D the leaves were treated with cytokinins at 4.5 μM in combination with naphthalenacetic acid (NAA) at 0.5 or 5 μM. At the 9th day of culture, the leaves from each treatment were transferred to a culture medium without growth regulators (GR-) or containing BA 2.66 μM + indole-3-butyric acid (IBA) 0.30 μM + gibberellic acid (GA3) 0.58 μM (GR+). Regeneration response was assessed after 52 days of culture. When 0.5 μM NAA was used, Kinetin treatment induced the highest formation of somatic embryos, particularly when the leaves were transferred to GR+ culture medium. TDZ exerted a pronounced positive effect on shoot production, while it strongly reduced somatic embryos and totally inhibited root regeneration. BA determined intermediate responses on all morphogenic structures regenerated. The increase of NAA concentration from 0.5 μM to 5.0 μM in the combination with Kin resulted in a decrease of somatic embryo-regenerating leaves both on GR- and GR+, while root-forming leaves increased on GR-. With TDZ, the higher concentration of NAA reduced the shoot-regenerating leaves, and exerted positive effects on somatic embryo- and root-regenerating leaves, which were very few or completely absent with NAA 0.5 μM.

Somatic embryo, adventitious root and shoot regeneration in in vitro grown quince leaves as influenced by treatments of different lenght with growth regulators

D'ONOFRIO, CLAUDIO;MORINI, STEFANO
2006

Abstract

The purpose of the work was to compare the effects of Kinetin (Kin), N 6-benzylaminopurine (BA) and Thidiazuron (TDZ) on somatic embryo, adventitious root and shoot regeneration from in vitro-cultured quince (Cydonia oblonga Mill.) leaves collected from the apical portion of actively proliferating shoots. After a two-day treatment with 2,4-D the leaves were treated with cytokinins at 4.5 μM in combination with naphthalenacetic acid (NAA) at 0.5 or 5 μM. At the 9th day of culture, the leaves from each treatment were transferred to a culture medium without growth regulators (GR-) or containing BA 2.66 μM + indole-3-butyric acid (IBA) 0.30 μM + gibberellic acid (GA3) 0.58 μM (GR+). Regeneration response was assessed after 52 days of culture. When 0.5 μM NAA was used, Kinetin treatment induced the highest formation of somatic embryos, particularly when the leaves were transferred to GR+ culture medium. TDZ exerted a pronounced positive effect on shoot production, while it strongly reduced somatic embryos and totally inhibited root regeneration. BA determined intermediate responses on all morphogenic structures regenerated. The increase of NAA concentration from 0.5 μM to 5.0 μM in the combination with Kin resulted in a decrease of somatic embryo-regenerating leaves both on GR- and GR+, while root-forming leaves increased on GR-. With TDZ, the higher concentration of NAA reduced the shoot-regenerating leaves, and exerted positive effects on somatic embryo- and root-regenerating leaves, which were very few or completely absent with NAA 0.5 μM.
D'Onofrio, Claudio; Morini, Stefano
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/199176
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