The aim of this research was to isolate protoplasts from in vitro-grown quince BA 29 leaves. Effects of various concentrations of cellulase and pectinase and different incubation periods were evaluated. The most effective treatment included (per ml) 50 units cellulase and 10 units pectinase, in the incubation medium and incubation for 21 h at 50 rpm in the dark at 27°C. This treatment produced a protoplast yield of 8.2 x 107 gfw-1 of which 90% were viable. Protoplast viability was influenced by length of the incubation period and ranged from about 40 to 100% among the various experiments.
Isolation of protoplasts from in vitro-grown quince BA29 leaves
D'ONOFRIO, CLAUDIO;MORINI, STEFANO;
1999-01-01
Abstract
The aim of this research was to isolate protoplasts from in vitro-grown quince BA 29 leaves. Effects of various concentrations of cellulase and pectinase and different incubation periods were evaluated. The most effective treatment included (per ml) 50 units cellulase and 10 units pectinase, in the incubation medium and incubation for 21 h at 50 rpm in the dark at 27°C. This treatment produced a protoplast yield of 8.2 x 107 gfw-1 of which 90% were viable. Protoplast viability was influenced by length of the incubation period and ranged from about 40 to 100% among the various experiments.File in questo prodotto:
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