3-Iodothyronamine (T1AM) is a novel relative of thyroid hormone that plays a role in critical body regulatory processes, such as glucose metabolism, thermal regulation and heart beating. This work was aimed at characterizing time dynamics of T1AM and its catabolite 3-iodothyroacetic acid (TA1) in different biological scales with LTI (Linear Time Invariant) models. Culture medium samples coming from culture of H9c2 murine cells and perfusion liquid samples from perfused rat heart were collected after the injection of a T1AM bolus. T1AM and TA1 concentrations in the samples were assayed with high performance liquid chromatography coupled to tandem mass spectrometry. Kinetic constants relative to T1AM transport and conversion were estimated with Weighted Least Squares method. We found that these constants can be related with an allometric power law depending on mass, with a negative exponent of -0.27± 0.19, implying that the velocity of conversion and internalization of T1AM decrease with increasing of system mass.

LTI Models for 3-Iodothyronamine Time Dynamics: A Multiscale View.

FRASCARELLI, SABINA;ZUCCHI, RICCARDO;VOZZI, GIOVANNI
2011

Abstract

3-Iodothyronamine (T1AM) is a novel relative of thyroid hormone that plays a role in critical body regulatory processes, such as glucose metabolism, thermal regulation and heart beating. This work was aimed at characterizing time dynamics of T1AM and its catabolite 3-iodothyroacetic acid (TA1) in different biological scales with LTI (Linear Time Invariant) models. Culture medium samples coming from culture of H9c2 murine cells and perfusion liquid samples from perfused rat heart were collected after the injection of a T1AM bolus. T1AM and TA1 concentrations in the samples were assayed with high performance liquid chromatography coupled to tandem mass spectrometry. Kinetic constants relative to T1AM transport and conversion were estimated with Weighted Least Squares method. We found that these constants can be related with an allometric power law depending on mass, with a negative exponent of -0.27± 0.19, implying that the velocity of conversion and internalization of T1AM decrease with increasing of system mass.
Orsi, G; Frascarelli, Sabina; Zucchi, Riccardo; Vozzi, Giovanni
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/200360
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