The benzodiazepine receptors of bovine brain membranes have been identified by the specific binding of radiolabeled [3H]diazepam. Pretreatment of membranes with N-ethylmaleimide causes a dose- and time-dependent decrease of 45 to 60% in the number of binding sites. No decrease occurs when membranes are pretreated with N-ethylmaleimide before administration, or in the presence, of diazepam. Binding of [3H]diazepam to the remaining sites occurs with the same characteristics as binding to the untreated receptor population.
|Autori:||MARTINI C; LUCACCHINI A|
|Titolo:||INACTIVATION OF BENZODIAZEPINE BINDING-SITES BY N-ETHYLMALEIMIDE|
|Anno del prodotto:||1982|
|Digital Object Identifier (DOI):||10.1111/j.1471-4159.1982.tb06661.x|
|Appare nelle tipologie:||1.1 Articolo in rivista|