The effect of seven light qualities (dark, far-red, far-red+blue, blue, white, red+blue, red) on induction and growth of MM106 apple callus cultures was studied comparatively. Internodes, collected from in vitro cultures grown under white light, were cultured under the various light qualities on gelled B5 medium supplemented with 6.8 M 2,4-D and 0.9 M BA. After 42 days, calluses were separated from the explants and placed under the same light qualities and growth medium. Callus cultures from two subsequent experiments were grown up to 112 and 126 days, respectively. Every 14 days, the cultures were weighed and transferred to fresh medium. At the end of these experiments another one was performed to test the effect of red, blue and white lights on some characteristics of cells grown in liquid media with the same chemical composition. The fresh weight of calluses appeared to be strongly increased by red light but inhibited by dark and blue light. Callus growth was also stimulated in the cultures grown under far-red light which differentiated friable and actively growing callus masses. Such cultures, after about two months of growth, showed fresh weight values not statistically different from those induced by red+blue light. In cell suspension cultures, cell size and shape appeared to be differently influenced by the various lights applied. When cells were transferred to growth medium without 2,4-D, formation of some nodules was observed regardless of light treatment. Histological analysis showed these nodules to have an organized structure, but regeneration was never observed during the experiment.
EFFECTS OF LIGHT QUALITY ON INDUCTION AND GROWTH OF APPLE MM106 CALLUS CULTURE
D'ONOFRIO, CLAUDIO;MORINI, STEFANO
2002-01-01
Abstract
The effect of seven light qualities (dark, far-red, far-red+blue, blue, white, red+blue, red) on induction and growth of MM106 apple callus cultures was studied comparatively. Internodes, collected from in vitro cultures grown under white light, were cultured under the various light qualities on gelled B5 medium supplemented with 6.8 M 2,4-D and 0.9 M BA. After 42 days, calluses were separated from the explants and placed under the same light qualities and growth medium. Callus cultures from two subsequent experiments were grown up to 112 and 126 days, respectively. Every 14 days, the cultures were weighed and transferred to fresh medium. At the end of these experiments another one was performed to test the effect of red, blue and white lights on some characteristics of cells grown in liquid media with the same chemical composition. The fresh weight of calluses appeared to be strongly increased by red light but inhibited by dark and blue light. Callus growth was also stimulated in the cultures grown under far-red light which differentiated friable and actively growing callus masses. Such cultures, after about two months of growth, showed fresh weight values not statistically different from those induced by red+blue light. In cell suspension cultures, cell size and shape appeared to be differently influenced by the various lights applied. When cells were transferred to growth medium without 2,4-D, formation of some nodules was observed regardless of light treatment. Histological analysis showed these nodules to have an organized structure, but regeneration was never observed during the experiment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
