We report the convenient use of 96-well microtiter plates in monitoring kidney recipient follow up by the measurement of urinary enzyme excretion rates: both the brush-border alanine aminopeptidase (AAP)and the lysosomal N-acetyl-beta-D-glucosaminidase (NAG) were assayed. Precisione and accuracy of enzyme activity determination were sufficiently preserved to advise this methodology when processing large number of samples.
The use of microtiter plates in the assay of urinary enzymes NAG and AAP: a useful time-and reagent-sparing procedure.
MATTEUCCI, ELENA;GIAMPIETRO, OTTAVIO
1996-01-01
Abstract
We report the convenient use of 96-well microtiter plates in monitoring kidney recipient follow up by the measurement of urinary enzyme excretion rates: both the brush-border alanine aminopeptidase (AAP)and the lysosomal N-acetyl-beta-D-glucosaminidase (NAG) were assayed. Precisione and accuracy of enzyme activity determination were sufficiently preserved to advise this methodology when processing large number of samples.File in questo prodotto:
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