Mycobacterium tuberculosis complex isolates (n=248), collected during a 1-year period in Tuscany, Italy, were genotyped for the katG463/gyrA95 polymorphism and by the standard spoligotyping and IS6110-RFLP assays. Most of the isolates (n=212, 85.5%) belonged to genotypic groups 2 and 3, which included most isolates from Italian-born patients. The remaining isolates belonged to group 1 organisms that were prevalent among foreign-born patients (29 out of 36, 80.6%). Spoligotype analysis detected 116 unique patterns and 34 clusters including 166 isolates. The combination of spoligotyping and IS6110-RFLP yielded 28 distinct clusters including 65 identical isolates (26.2%); 22 clusters were constituted by 2 isolates, 4 clusters by 3 isolates and 2 clusters by 4 and 5 isolates each, thus proving a low transmission rate in the community. Predominant spoligotypes yielding 50% of clustered isolates were found in 6 clusters that included the widespread type 53 (clade T1) with 29 isolates (11.7% of total isolates), types 50 and 47 (Haarlem family) with 18 (7.3%) and 8 (3.2%) isolates respectively, type 42 (Latino-American and Mediterranean clade) with 13 isolates (5.2%), the new type 1737 (named "Tuscany") with 8 isolates (3.2%), and type 1 (W-Beijing family) with 7 isolates (2.8%). Other spoligotype families, such as the Mycobacterium africanum, East-African Indian (EAI2/Manila), and Central Asia 1 (CAS1/Delhi) families, all including organisms of genotypic group 1, as well as the Cameroun family (genotypic group 2), were detected especially among immigrant patients. The occurrence of genotypes originally found in distant geographic areas with high prevalence of tuberculosis may represent an hallmark for changes in transmission dynamics of tuberculosis in the region in the near future.
|Autori:||Lari N; Rindi L; Sola C; Bonanni D; Rastogi N; Tortoli E; Garzelli C|
|Titolo:||Genetic Diversity, Determined on the Basis of katG463 and gyrA95 Polymorphisms, Spoligotyping, and IS6110 Typing, of Mycobacterium tuberculosis Complex Isolates from Italy|
|Anno del prodotto:||2005|
|Digital Object Identifier (DOI):||10.1128/JCM.43.4.1617-1624.2005|
|Appare nelle tipologie:||1.1 Articolo in rivista|