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EnglishA collection of clinical isolates including 9 Mycobacterium bovis bacille Calmette-Guérin (BCG), 37 M. bovis and 1 isolate identified as M. bovis/caprae intermediate, recovered from humans in Tuscany, Italy, from 1990 to 2009, was genotyped by spoligotyping and Variable Number Tandem Repeat (VNTR) typing. Spoligotyping detected 15 unique profiles; the "BCG-like" SIT482/SB0120 spoligotype was largely prevalent accounting for 63.8% of isolates. VNTR typing, based on the 15 VNTR loci commonly tested for Mycobacterium tuberculosis, detected 29 unique profiles; only 8 VNTR loci (VNTR 43, MIRU 04, QUB-11b, ETR-A, VNTR 47, MIRU 31, QUB-26 and VNTR 53) provided a satisfactory allelic diversity in the VNTR analysis. Combined together, spoligotyping and VNTR typing yielded 33 unique patterns and 5 clusters including a total of 19 isolates. Clustered isolates, further typed for additional 9 VNTR loci, finally yielded 3 distinct clusters including 3 M. bovis BCG isolates each, and 1 cluster of 6 M. bovis isolates. Minimum spanning tree analysis showed that, in spite of the many distinct VNTR profiles, most M. bovis isolates displayed a high phylogenetic proximity, due to the variation of a single VNTR allele, thus indicating that the population of human M. bovis isolates in our setting is relatively homogeneous and conserved.
| Autori interni: | |
| Autori: | Lari N; Bimbi N; Rindi L; Tortoli E; Garzelli C |
| Titolo: | Genetic diversity of human isolates of Mycobacterium bovis assessed by Spoligotyping and Variable-Number-Tandem-Repeat genotyping |
| Anno del prodotto: | 2011 |
| Digital Object Identifier (DOI): | 10.1016/J.MEEGID.2010.09.004. |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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