In our previous study, RAPD (Random Amplified Polymorphic DNA) analysis revealed species-specific markers for three medicinal Echinacea species (Asteraceae): E. angustifolia DC., E. pallida (Nutt.) Nutt. and E. purpurea (L.) Moench. In the present work, we have converted a RAPD marker (750 bp) for E. purpurea into a SCAR (Sequence Characterized Amplified Region) marker. SCAR-PCR, in fact, revealed the expected amplicon (330 bp) only in E. purpurea and not in the other two species, giving further evidence for differences in medicinal Echinacea spp. genome and confirming a greater similarity between E. pallida and angustifolia.
Autori interni: | |
Autori: | Adinolfi, Barbara; Chicca, A; Martinotti, E; Breschi, MARIA CRISTINA; Nieri, Paola |
Titolo: | Sequence characterized amplified region (SCAR) analysis on DNA from the three medicinal Echinacea species |
Anno del prodotto: | 2007 |
Digital Object Identifier (DOI): | 10.1016/j.phytochem.2006.05.006 |
Appare nelle tipologie: | 1.1 Articolo in rivista |