Grapevine rupestris stem pitting associated virus (GRSPaV), is the putative causal agent of rupestris stem pitting (RSP) a component of the rugose wood disease of grapevine. Recently, a strikingly high association has been detected between GRSPaV and vein necrosis (VN), suggesting that 110 Richter (110 R) is the best indicator of GRSPaV infection. In the present study, we investigate the occurrence of molecular variants of GRSPaV in grapevine accessions previously indexed for vein necrosis on 110 R. 100% of the VN infected accessions results positive to GRSPaV by RT-PCR using primers 13/14. Using group specific primers, group I was detected in all the 22 accessions infected with VN, 11 times in mix infection with group III, 3 times with group II, and twice as a single infection. Unexpected results were observed with group II, in fact this group is present in 6 accessions negative to VN and to GRSPaV with primers 13/14. No single infection was, however, recorded for group III, the only available indication is its exclusive presence in VN infected accessions. This work confirms the association between GRSPaV and vein necrosis.
Study of the Relationship Between Grapevine Ruspestris Stem Pitting Associated Virus Molecular Variants and Vein Necrosis
MATERAZZI, ALBERTO;TRIOLO, ENRICO
2006-01-01
Abstract
Grapevine rupestris stem pitting associated virus (GRSPaV), is the putative causal agent of rupestris stem pitting (RSP) a component of the rugose wood disease of grapevine. Recently, a strikingly high association has been detected between GRSPaV and vein necrosis (VN), suggesting that 110 Richter (110 R) is the best indicator of GRSPaV infection. In the present study, we investigate the occurrence of molecular variants of GRSPaV in grapevine accessions previously indexed for vein necrosis on 110 R. 100% of the VN infected accessions results positive to GRSPaV by RT-PCR using primers 13/14. Using group specific primers, group I was detected in all the 22 accessions infected with VN, 11 times in mix infection with group III, 3 times with group II, and twice as a single infection. Unexpected results were observed with group II, in fact this group is present in 6 accessions negative to VN and to GRSPaV with primers 13/14. No single infection was, however, recorded for group III, the only available indication is its exclusive presence in VN infected accessions. This work confirms the association between GRSPaV and vein necrosis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
