DNA synthesis of human hepatoblastoma HepG2 cells is reversibly inhibited by butyrate. When butyrate is removed from the culture medium, cells re-enter the cell cycle, synthesizing DNA with a time lag of about 12 h. HepG2 cells, growth-inhibited for 30 h with butyrate, synthesize and accumulate a nuclear protein, called D. Protein D synthesis is inhibited in cells which, released from the butyrate block, have resumed DNA synthesis as well as in growing cells never exposed to butyrate. Protein D has been purified from growth-arrested cells and partially sequenced. The amino acid sequences of five internal trypsin peptides indicate that protein D is a novel nuclear protein.
|Autori:||DONADEL G; GARZELLI C; FRANK R; GABRIELLI F|
|Titolo:||IDENTIFICATION OF A NOVEL NUCLEAR-PROTEIN SYNTHESIZED IN GROWTH-ARRESTED HUMAN HEPATOBLASTOMA HEPG2 CELLS|
|Anno del prodotto:||1991|
|Digital Object Identifier (DOI):||10.1111/j.1432-1033.1991.tb15759.x|
|Appare nelle tipologie:||1.1 Articolo in rivista|