The possibility of realizing bioartificial polymeric materials from blends of the enzyme alpha-amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of him and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X-ray diffractometry and morphological analysis. A certain degree of interaction through which the alpha-amylase molecules influence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary significantly. In addition, hydrogels prepared by a freeze-thawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity.
Enzyme-based bioartificial polymeric materials. The alpha-amylase-poly(vinyl alcohol) system
LAZZERI, LUIGI;CASCONE, MARIA GRAZIA;POLACCO, GIOVANNI;
1997
Abstract
The possibility of realizing bioartificial polymeric materials from blends of the enzyme alpha-amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of him and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X-ray diffractometry and morphological analysis. A certain degree of interaction through which the alpha-amylase molecules influence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary significantly. In addition, hydrogels prepared by a freeze-thawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
