Peripheral nerves may be severely compromised as a consequence of mechanical trauma, especially when they present a total disruption of the nerve (neurotmesis). The difficulty that we meet during the surgical realignment of the proximal and distal nerve ends are: scar reaction around the nerve, the granulomatous reaction (result of micro-surgically suture), the blood or other material interposition along the regenerated nerve, a careful alignment of both nerve stumps and the tension at the coaptation site that could be created in a large gap. In order to obtain a perfect and functional alignment of neuronal fibres different techniques for bridging defects in peripheral nerve have been developed, in particular the implant of biodegradable polymeric guidance channels has shown considerable results. In this study preliminarily polyurethane-based nerve guide have been realised whit an extrusion system and then filled with specific cell adhesion biomolecules (gelatin and polylisine). This guides have been tested in vitro and in vivo. In particular during in-vivo experiments an accurate electromyography on the rat before the explant and histology of explanted tube were performed. These results have been compared with those obtained using polycaprolactone tubes, that is a bioerodable polymer approved by FDA and used in several commercial products. The results have shown a good regeneration either in vitro and in vivo with an appreciable restore of neural tissue function.

L'"EFFETTO TUNNEL" MEDIANTE TUBI BIOERODIBILE NELLA NERVE REGENERATION

CARLUCCI, FABIO;VOZZI, GIOVANNI;VOZZI, FEDERICO;SALVADORI, CLAUDIA;
2005

Abstract

Peripheral nerves may be severely compromised as a consequence of mechanical trauma, especially when they present a total disruption of the nerve (neurotmesis). The difficulty that we meet during the surgical realignment of the proximal and distal nerve ends are: scar reaction around the nerve, the granulomatous reaction (result of micro-surgically suture), the blood or other material interposition along the regenerated nerve, a careful alignment of both nerve stumps and the tension at the coaptation site that could be created in a large gap. In order to obtain a perfect and functional alignment of neuronal fibres different techniques for bridging defects in peripheral nerve have been developed, in particular the implant of biodegradable polymeric guidance channels has shown considerable results. In this study preliminarily polyurethane-based nerve guide have been realised whit an extrusion system and then filled with specific cell adhesion biomolecules (gelatin and polylisine). This guides have been tested in vitro and in vivo. In particular during in-vivo experiments an accurate electromyography on the rat before the explant and histology of explanted tube were performed. These results have been compared with those obtained using polycaprolactone tubes, that is a bioerodable polymer approved by FDA and used in several commercial products. The results have shown a good regeneration either in vitro and in vivo with an appreciable restore of neural tissue function.
Carlucci, Fabio; Dini, F; Vozzi, Giovanni; Vozzi, Federico; Chiono, V; Salvadori, Claudia; Arispici, M; Domenici, C; Ciardelli, G; Giusti, P.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/204397
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