Real-time PCR (RT-PCR) is a sensitive technique that has been recently applied to the determination and quantification of fetomaternal microchimerism.1–3 In a previous manuscript, we detected the presence of Y chromosome sequences in female patients with systemic lupus erythaematosus (SLE) using nested PCR.4 In the present study we evaluated the presence of Y chromosome sequences in the peripheral blood mononuclear cells (PBMCs) of female patients with systemic sclerosis (SSc) using nested and RT-PCR, correlating the results with the clinical and serological manifestations of the patients.
|Autori:||MOSCA M; GIULIANO T; CURCIO M; DOVERI M; DE FEO F; TANI C; BAZZICHI L; BOMBARDIERI S|
|Titolo:||Comparison of real-time PCR and nested PCR for the detection of Y chromosome sequences in the peripheral blood mononuclear cells of patients with systemic sclerosis|
|Anno del prodotto:||2009|
|Appare nelle tipologie:||1.1 Articolo in rivista|