Recent studies suggest that anti-DNA antibodies may arise from the immune response to a complex of DNA and a DNA-binding protein. One of the protein targets frequently recognized by anti-DNA antibodies is the enzyme DNase I. To investigate the possible role of DNase I in the induction of anti-DNA antibodies, we immunized preautoimmune NZBxNZW F1 mice with a complex of DNA and DNase I emulsified in complete Freund's adjuvant. Control mice received DNA or DNase in adjuvant. IgG anti-dsDNA antibodies were induced in 50% of the mice immunized with DNA-DNase, in 25% of the mice immunized with DNase and in 6% of the mice immunized with DNA. However, immunized mice that produced anti-DNA antibodies did not develop renal disease. These data show that a DNA-binding protein like DNase may act as carrier in the immune response that leads to anti-DNA antibodies production in an autoimmune strain, but the induced anti-dsDNA antibodies have a low pathogenic potential.

Induction of anti-DNA antibodies in pre-autoimmune NZBxNZW F1 mice by immunization with a DNA-DNase I complex

MARCHINI, BARBARA;BOMBARDIERI, STEFANO;MIGLIORINI, PAOLA
2000

Abstract

Recent studies suggest that anti-DNA antibodies may arise from the immune response to a complex of DNA and a DNA-binding protein. One of the protein targets frequently recognized by anti-DNA antibodies is the enzyme DNase I. To investigate the possible role of DNase I in the induction of anti-DNA antibodies, we immunized preautoimmune NZBxNZW F1 mice with a complex of DNA and DNase I emulsified in complete Freund's adjuvant. Control mice received DNA or DNase in adjuvant. IgG anti-dsDNA antibodies were induced in 50% of the mice immunized with DNA-DNase, in 25% of the mice immunized with DNase and in 6% of the mice immunized with DNA. However, immunized mice that produced anti-DNA antibodies did not develop renal disease. These data show that a DNA-binding protein like DNase may act as carrier in the immune response that leads to anti-DNA antibodies production in an autoimmune strain, but the induced anti-dsDNA antibodies have a low pathogenic potential.
D., Chimenti; Marchini, Barbara; S., Manzini; Bombardieri, Stefano; Migliorini, Paola
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/205039
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