The presence of a [3H]muscimol binding site on the purified benzodiazepine receptor was demonstrated. The purified protein was apparently homogeneous as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis (stained with silver), with a molecular weight of 60,000 +/- 3000. The benzodiazepine binding sites were characterized as being of the central type and the [3H]flunitrazepam binding was enhanced by GABA. This activation was antagonized by bicuculline. [3H]Muscimol specifically binds to the benzodiazepine receptor. The Scatchard plot indicates a Kd of 23 nM and the ratio [3H]flunitrazepam/[3H]muscimol is approximately unity.
|Autori:||Martini C; Rigacci T; Lucacchini A.|
|Titolo:||[H-3]MUSCIMOL BINDING-SITE ON PURIFIED BENZODIAZEPINE RECEPTOR|
|Anno del prodotto:||1983|
|Digital Object Identifier (DOI):||10.1111/j.1471-4159.1983.tb09070.x|
|Appare nelle tipologie:||1.1 Articolo in rivista|