Macrophage NO2- production as a sensitive and rapid assay for the quantitation of murine IFN-gamma

Macrophage production of arginine-derived NO2- provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO2- release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO2- production assay.



Autori interni: 
MIGLIORINI, PAOLA
mostra contributor esterni 
Autori: Migliorini P; Corradin G; Corradin SB
Titolo: Macrophage NO2- production as a sensitive and rapid assay for the quantitation of murine IFN-gamma
Anno del prodotto: 1991
Appare nelle tipologie:1.1 Articolo in rivista

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Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/11568/20629
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