Neurotransmitters play important roles in neurogenesis; in particular, acetylcholine and serotonin may regulate neurite elongation. Acetylcholine may also activate transcription factors such as EGR-1, which plays a role in neurite extension. N18TG2 neuroblastoma cells were transfected with constructs containing the genes coding for choline acetyltransferase, 5-HT1A and 5-HT2B serotonin receptors to study acetylcholine and serotonin interplay in neurite outgrowth. 5-HT1A receptor stimulation causes a decrease in EGR-1 levels and inhibition of neurite outgrowth; 5-HT2B stimulation has instead no effect. Cholinergic stimulation, on the other end, increases EGR-1 levels and fiber outgrowth. When both cholinergic and 5-HT1A receptors are stimulated, fiber outgrowth is restored; therefore acetylcholine counterbalances the inhibitory effect of serotonin on neurite outgrowth. These results suggest that EGR-1 plays a role in the interplay of acetylcholine and serotonin in the regulation of neurite extension during development.
5-HT1A and 5-HT2B serotonin receptors in neurite outgrowth: involvement of EGR-1
ORI, MICHELA;
2013-01-01
Abstract
Neurotransmitters play important roles in neurogenesis; in particular, acetylcholine and serotonin may regulate neurite elongation. Acetylcholine may also activate transcription factors such as EGR-1, which plays a role in neurite extension. N18TG2 neuroblastoma cells were transfected with constructs containing the genes coding for choline acetyltransferase, 5-HT1A and 5-HT2B serotonin receptors to study acetylcholine and serotonin interplay in neurite outgrowth. 5-HT1A receptor stimulation causes a decrease in EGR-1 levels and inhibition of neurite outgrowth; 5-HT2B stimulation has instead no effect. Cholinergic stimulation, on the other end, increases EGR-1 levels and fiber outgrowth. When both cholinergic and 5-HT1A receptors are stimulated, fiber outgrowth is restored; therefore acetylcholine counterbalances the inhibitory effect of serotonin on neurite outgrowth. These results suggest that EGR-1 plays a role in the interplay of acetylcholine and serotonin in the regulation of neurite extension during development.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
