SFX Get it!(opens in a new window)|View at Publisher| Export | Download | Add to List | More... European Journal of Pharmaceutical Sciences Volume 50, Issue 3-4, 2013, Pages 393-399 Delivery of natural polyphenols by polymeric nanoparticles improves the resistance of endothelial progenitor cells to oxidative stress (Article) Felice, F.a , Zambito, Y.b, Belardinelli, E.b, D'Onofrio, C.c, Fabiano, A.b, Balbarini, A.a, Di Stefano, R.a a Department of Surgery, Medical, Molecular and Critical Area Pathology, University of Pisa, via Pardisa, 2, 56124 Pisa, Italy b University of Pisa, Department of Pharmacy, via Bonnano, 33, Pisa, Italy c University of Pisa, Department of Cultivation and Protection of Ligneous Species G. Scaramuzzi, Via del Borghetto, 80, Pisa, Italy View additional affiliations View references (27) Abstract Purpose Bone marrow-derived endothelial progenitor cells (EPCs) circulate into peripheral blood and significantly contribute to neo-vascularisation and re-endothelialisation as part of the process of vascular repair. Several studies have reported decreased EPC number in the presence of oxidative stress. Aim of this study was to evaluate the validity of mucoadhesive polymeric nanoparticles as a delivery system of natural products able to protect EPCs from oxidative stress. Methods The total polyphenol content and antioxidant capacity of red grape seed extract (GSE) either pre-veraison (p-GSE) or ripe (r-GSE) were measured. Cell viability was evaluated by WST-1 assay. Nanoparticles were prepared by ionotropic crosslinking of two structurally different thiolated quaternary ammonium-chitosan conjugates. A hyaluronic acid solution, containing p-GSE or r-GSE, was added to a stirred solution of each of the two chitosan derivatives to obtain p- or r-GSE loaded nanoparticles (NP) of two types. Results Both GSE types demonstrated strong antioxidant capacity. p-GSE showed a higher content in total polyphenols compared to r-GSE. NP size was in the 310-340 nm range, with 24 h stability, and nearly 100% encapsulation efficiency for both GSE types. NP were internalized by cells to an extent related directly with their surface charge intensity. GSE-NP uptake significantly improved cell viability and resistance to oxidation. Conclusions Nanotechnology has a great potential in nutraceutical delivery. The present results suggest that NP is a highly promising polyphenol carrier system particularly useful to protect EPCs from oxidative stress, thus improving their survival.
Delivery of natural polyphenols by polymeric nanoparticles improves the resistance of endothelial progenitor cells to oxidative stress
FELICE, FRANCESCA;ZAMBITO, YLENIA;D'ONOFRIO, CLAUDIO;Fabiano A;BALBARINI, ALBERTO;DI STEFANO, ROSSELLA
2013-01-01
Abstract
SFX Get it!(opens in a new window)|View at Publisher| Export | Download | Add to List | More... European Journal of Pharmaceutical Sciences Volume 50, Issue 3-4, 2013, Pages 393-399 Delivery of natural polyphenols by polymeric nanoparticles improves the resistance of endothelial progenitor cells to oxidative stress (Article) Felice, F.a , Zambito, Y.b, Belardinelli, E.b, D'Onofrio, C.c, Fabiano, A.b, Balbarini, A.a, Di Stefano, R.a a Department of Surgery, Medical, Molecular and Critical Area Pathology, University of Pisa, via Pardisa, 2, 56124 Pisa, Italy b University of Pisa, Department of Pharmacy, via Bonnano, 33, Pisa, Italy c University of Pisa, Department of Cultivation and Protection of Ligneous Species G. Scaramuzzi, Via del Borghetto, 80, Pisa, Italy View additional affiliations View references (27) Abstract Purpose Bone marrow-derived endothelial progenitor cells (EPCs) circulate into peripheral blood and significantly contribute to neo-vascularisation and re-endothelialisation as part of the process of vascular repair. Several studies have reported decreased EPC number in the presence of oxidative stress. Aim of this study was to evaluate the validity of mucoadhesive polymeric nanoparticles as a delivery system of natural products able to protect EPCs from oxidative stress. Methods The total polyphenol content and antioxidant capacity of red grape seed extract (GSE) either pre-veraison (p-GSE) or ripe (r-GSE) were measured. Cell viability was evaluated by WST-1 assay. Nanoparticles were prepared by ionotropic crosslinking of two structurally different thiolated quaternary ammonium-chitosan conjugates. A hyaluronic acid solution, containing p-GSE or r-GSE, was added to a stirred solution of each of the two chitosan derivatives to obtain p- or r-GSE loaded nanoparticles (NP) of two types. Results Both GSE types demonstrated strong antioxidant capacity. p-GSE showed a higher content in total polyphenols compared to r-GSE. NP size was in the 310-340 nm range, with 24 h stability, and nearly 100% encapsulation efficiency for both GSE types. NP were internalized by cells to an extent related directly with their surface charge intensity. GSE-NP uptake significantly improved cell viability and resistance to oxidation. Conclusions Nanotechnology has a great potential in nutraceutical delivery. The present results suggest that NP is a highly promising polyphenol carrier system particularly useful to protect EPCs from oxidative stress, thus improving their survival.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
