Abstract MYB factors comprise one of the largest family of transcription factors (TFs) characterised by a highly conserved DNA-binding domain: the MYB domain. From sunflower genome, we have isolated two R2R3-type MYB TFs (Ha-R2R3-MYB1 and Ha-R2R3-MYB2) that show homology with the functional domain of most R2R3-MYB proteins of other species. The R2 (53 amino acids) and R3 (51 amino acids) motifs of sunflower MYB TFs contain typical amino acids, including a series of highly conserved tryptophan residues, which play a key role in sequence-specific DNA binding. In the MYB domain, both genes (Ha-R2R3-MYB1 and Ha-R2R3-MYB2) exhibit the highly conserved splicing arrangement of three exons and two introns. Using in situ hybridization, a weak-level of Ha-R2R3-MYB1 transcription was uniformly spread in shoot apical meristem (SAM) as well as in axillary meristem (AM). By contrast, Ha-R2R3-MYB2 transcription was strongly restricted to a small domain within the boundary zone separating the SAM and the leaf primordia, suggesting that Ha-R2R3-MYB2 may be involved in an early step of AM development.

Isolation and molecular analysis of two R2R3-MYB genes from sunflower (Helianthus annuus)

PUGLIESI, CLAUDIO;FAMBRINI, MARCO
2013

Abstract

Abstract MYB factors comprise one of the largest family of transcription factors (TFs) characterised by a highly conserved DNA-binding domain: the MYB domain. From sunflower genome, we have isolated two R2R3-type MYB TFs (Ha-R2R3-MYB1 and Ha-R2R3-MYB2) that show homology with the functional domain of most R2R3-MYB proteins of other species. The R2 (53 amino acids) and R3 (51 amino acids) motifs of sunflower MYB TFs contain typical amino acids, including a series of highly conserved tryptophan residues, which play a key role in sequence-specific DNA binding. In the MYB domain, both genes (Ha-R2R3-MYB1 and Ha-R2R3-MYB2) exhibit the highly conserved splicing arrangement of three exons and two introns. Using in situ hybridization, a weak-level of Ha-R2R3-MYB1 transcription was uniformly spread in shoot apical meristem (SAM) as well as in axillary meristem (AM). By contrast, Ha-R2R3-MYB2 transcription was strongly restricted to a small domain within the boundary zone separating the SAM and the leaf primordia, suggesting that Ha-R2R3-MYB2 may be involved in an early step of AM development.
Pugliesi, Claudio; Salvini, M.; Fambrini, Marco
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/253774
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