The dermatophyte Microsporum cookei Ajello was treated with nine new natural and synthetic 2,2': 5',2''-terthiophenes to determine their possible antifungal activity. In the dark the thiophenes were inactive, while when photoactivated with UV-A they induced a remarkable reduction in the growth rate of the fungus. The only exception was (E)-N-(2-methylpropyl)-3-(2,2': 5',2''-terthien-5-yl)-propenamide, which was not fungistatic even at the highest dose tested (24 mu M). The more active compounds were 3'-methoxy-2,2' : 5',2''-terthiophene and 3'-methylthio-2,2': 5',2''-terthiophene, whose activity seems to be related to the presence of a substituent in the 3' position of the central ring of thiophenes. Transmission electron microscopic observations demonstrated the photoactive nature of the synthetic molecules to be similar to that of alpha-terthienyl, a natural thiophene present in some Asteraceae. The dark treatment caused only the accumulation of the compound in vacuoles, without other evident alterations. After UV-A irradiation the activated thiophene causes severe modifications to the endomembrane system, probably via oxygen-dependent mechanism.

Antifungal activity of some 2,2':5',2"-terthiophene derivatives

ROSSI, RENZO;CARPITA, ADRIANO;
1994

Abstract

The dermatophyte Microsporum cookei Ajello was treated with nine new natural and synthetic 2,2': 5',2''-terthiophenes to determine their possible antifungal activity. In the dark the thiophenes were inactive, while when photoactivated with UV-A they induced a remarkable reduction in the growth rate of the fungus. The only exception was (E)-N-(2-methylpropyl)-3-(2,2': 5',2''-terthien-5-yl)-propenamide, which was not fungistatic even at the highest dose tested (24 mu M). The more active compounds were 3'-methoxy-2,2' : 5',2''-terthiophene and 3'-methylthio-2,2': 5',2''-terthiophene, whose activity seems to be related to the presence of a substituent in the 3' position of the central ring of thiophenes. Transmission electron microscopic observations demonstrated the photoactive nature of the synthetic molecules to be similar to that of alpha-terthienyl, a natural thiophene present in some Asteraceae. The dark treatment caused only the accumulation of the compound in vacuoles, without other evident alterations. After UV-A irradiation the activated thiophene causes severe modifications to the endomembrane system, probably via oxygen-dependent mechanism.
Mares, D; Romagnoli, C; Rossi, Renzo; Carpita, Adriano; Ciofalo, M; Bruni, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/25810
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