In Glomus mosseae microtubules and microfilaments were visualized by indirect immunofluorescence microscopy in hyphae quick-frozen, freeze-substituted, and treated with cell-wall-degrading enzymes. Microtubules were distinguished in both the cortical and the central parts of hyphae, while microfilaments were revealed only in the cortical part. In immunoblotting, tubulin and actin were detected in an extract from hyphae elicited by host plants.
Cytoskeletal components in the arbuscular mycorrhizal fungus Glomus mosseae
GIOVANNETTI, MANUELA;
1994-01-01
Abstract
In Glomus mosseae microtubules and microfilaments were visualized by indirect immunofluorescence microscopy in hyphae quick-frozen, freeze-substituted, and treated with cell-wall-degrading enzymes. Microtubules were distinguished in both the cortical and the central parts of hyphae, while microfilaments were revealed only in the cortical part. In immunoblotting, tubulin and actin were detected in an extract from hyphae elicited by host plants.File in questo prodotto:
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