Previous studies have demonstrated the tumor-targeting potential of radioiodinated 5-iodo-2'-deoxyuridine (IUdR) in experimental animal models following direct intratumoral or intracavitary administration. The aim of this study was to measure the tumor uptake and metabolic fate of 5-[125I]iodo-2'-deoxyuridine ([125I]UdR) in humans after a single intratumoral injection. Ten patients with colorectal cancer were injected intratumorally with [125I]UdR) (0.24-3.9 MBq) during endoscopy 24 hr before ablative surgery. Blood and urine samples were collected up to 72 hr after [125I]UdR injection. Following resection, the radioactivity in the tumor and the surrounding tissues was measured in a gamma counter, and microautoradiography was performed on semi-thin tissue sections to assess localization of the radiopharmaceutical at the cellular level. An average of 0.234% of the injected dose was present per gram of tumor (range 0.009-0.918, median value 0.147), and tumor-to-nontumor radioactivity incorporation ratios were high for colonic mucosa when the nontumor tissue was taken at 1 cm (mean 629, range 27-2391) and 15 cm (mean 2387, range 122-12674) from the injection site. Microautoradiography confirmed these high tumor-to-nontumor ratios and demonstrated localization of [125I]UdR in the tumor cell nuclei. These results suggest that radioiodinated IUdR might have potential as a tumor-targeting agent in humans, provided homogeneous intratumoral distribution of the radiopharmaceutical by a suitable route of loco-regional administration can be achieved.
|Autori interni:||MARIANI, GIULIANO|
DI STEFANO, ROSSELLA
|Autori:||Mariani G; Cei A; Collecchi P; Baranowska-Kortylevicz J; Van Den Abbeele AD; Di Luca L; Di Stefano R; Viacava P; Ferdeghini M; Di Sacco S; Salvadori PA; Bevilacqua G; Adelstein SJ; Mosca F; Kassis AI|
|Titolo:||Tumor targeting in vivo and metabolic fate of 5-(Iodine-125)-Iodo-2’- Deoxyuridine following intratumoral injection in patients with colorectal cancer.|
|Anno del prodotto:||1993|
|Appare nelle tipologie:||1.1 Articolo in rivista|