Synthetic peptides have been used to map linear B-cell epitopes of the third variable (V3) region of the feline immunodeficiency virus (FIV) external membrane glycoprotein gp120. The analysis of sera from naturally and experimentally FIV-infected cats by Pepscan and enzyme immunoassay with four partially overlapping peptides evidenced three antibody-binding domains, two of which mapped in the carboxyl-terminal half of V3. In particular, the V3.3 sequence (Gly-392-Phe-413) turned out to be important for in vitro neutralization of the virus in that the peptide inhibited the FIV-neutralizing activity of pooled immune cat sera, and on the other hand, cat sera raised against this peptide effectively neutralized FIV infectivity for Crandell feline kidney cells.
|Autori:||LOMBARDI S; GARZELLI C; LAROSA C; ZACCARO L; SPECTER S; MALVALDI G; TOZZINI F; ESPOSITO F; BENDINELLI M|
|Titolo:||IDENTIFICATION OF A LINEAR NEUTRALIZATION SITE WITHIN THE 3RD VARIABLE REGION OF THE FELINE IMMUNODEFICIENCY VIRUS ENVELOPE|
|Anno del prodotto:||1993|
|Appare nelle tipologie:||1.1 Articolo in rivista|