Behcet's disease (BD) is a chronic multisystemic inflammatory disorder characterized mainly by recurrent oral and genital aphthous ulcerations and uveitis. Etiology and pathogenesis of BD remain unknown. T cell receptor (TCR) V alpha/V beta gene product expression as well as J beta gene segment expression in peripheral blood of BD patients were analysed to investigate the possible role of T lymphocytes in the etiopathogenesis of BD. Flow cytometry with 12 TCR V-specific MoAbs was used for TCRV analyses. J beta gene segment usage by T cell populations expressing certain V beta s was determined by polymerase chain reaction (PCR) technique with V beta- and C beta-specific primers, Southern blotting of PCR products, and subsequent hybridization with radiolabelled J beta gene segment-specific probes. Although 13 of the 23 BD patients exhibited increases in expression of one or more TCR V-gene products, only expansions among the CD4(+) T cell subset were significantly more frequent in BD patients (7/23) compared with healthy controls (0/15) (P = 0.019). Six out of eight cases followed for up to 20 months had at least one expansion correlated with disease activity. A strict preference for particular J beta gene segments implicating clonality was apparent in all analysed T cell expansions and correlated well with disease activity. These results suggest a possible involvement of antigen-specific T lymphocytes in the pathogenesis of BD.
|Autori:||Esin S; Gül A; Hodara V; Jeddi-Tehrani M; Dilsen N; Koniçe M; Andersson R; Wigzell H|
|Titolo:||Peripheral blood T cell expansions in patients with Behcet's disease|
|Anno del prodotto:||1997|
|Digital Object Identifier (DOI):||10.1046/j.1365-2249.1997.d01-947.x|
|Appare nelle tipologie:||1.1 Articolo in rivista|