We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.

Region-specific DNA alterations in focally induced seizures

Giorgi Filippo Sean;Frenzilli Giada;Scarcelli Vittoria;Nigro Marco;Bernardeschi Margherita;Fornai Francesco
2014

Abstract

We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.
Cantafora, Emanuela; Giorgi, FILIPPO SEAN; Frenzilli, Giada; Scarcelli, Vittoria; Busceti Carla, L; Nigro, Marco; Bernardeschi, Margherita; Fornai, Francesco
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/473870
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