Relative levels of expression of T cell receptor variable (V) beta and joining (J) beta gene segments were determined in T cells derived from intestinal biopsies of healthy mucosal areas, mesenteric lymph nodes and peripheral blood of the same individuals. Samples taken from patients suffering from inflammatory (n = 8) and non-inflammatory (n = 8) bowel diseases were analyzed by semi-quantitative polymerase chain reaction-based methods. In the intestine, fewer (median = 3.5) V beta gene segments constituted more than 50% of the T cell receptor V beta repertoire compared to that of peripheral blood T cells (median = 7, P < 0.001). Interestingly, in all sixteen individuals studied, intestinal T lymphocytes (IL-T) expressed the V beta 7 gene family to a higher degree than did T cells in the paired peripheral blood and mesenteric lymph nodes (P < 0.001). T cell receptor J beta gene segment analyses of V beta 7+ T cells revealed no significant difference in oligoclonality rates between peripheral blood (4/16) and intestine (7/16) (P = 0.46). Hence, overexpression of intestinal TCR V beta 7 message does not seem to be due to oligoclonal expansions in the majority of the samples.
|Autori interni:||ESIN, SEMIH|
|Autori:||Esin S; Hodara V; Jeddi-Tehrani M; Grunewald J; Svenberg T; Andersson R; Wigzell H|
|Titolo:||Enhanced prevalence of T cell receptor Vbeta7 gene family expression in human intestine-associated T lymphocytes|
|Anno del prodotto:||1996|
|Digital Object Identifier (DOI):||10.1016/0165-2478(96)02544-8|
|Appare nelle tipologie:||1.1 Articolo in rivista|