Surface topography of human chromosomes was examined by atomic force microscopy during treatments for G-banding. Trypsin treatment resulted in a structural modification in the chromatin. Subsequent Giemsa staining caused a general swelling of the chromosomal surface that was greater in the areas of G-band positive regions. By means of a quantitative evaluation method we showed that the G-banding process produces a 10-fold enhancement of a pre-existing pattern of chromatin between G-band positive and G-band negative regions on mitotic chromosomes.
|Autori:||MUSIO A; MARIANI T; FREDIANI C; ASCOLI C; SBRANA I|
|Titolo:||Atomic force microscope imaging of chromosome structure during G-banding treatments|
|Anno del prodotto:||1997|
|Digital Object Identifier (DOI):||10.1139/g97-018|
|Appare nelle tipologie:||1.1 Articolo in rivista|