tOpiates play a relevant role in forensic toxicology and their assay in urine or blood is usually performed forexample in workplace drug-testing or toxicological investigation of drug impaired driving. The presentwork describes two new methods for detecting morphine, codeine and 6-monoacethyl morphine inhuman urine or blood using a single step derivatisation in aqueous phase. Propyl chloroformate is used asthe dramatizing agent followed by liquid–liquid extraction and gas-chromatography–mass spectroscopyto detect the derivatives. The methods have been validated both for hydrolysed and unhydrolysed urine.For hydrolysed urine, the LOD and LOQ were 2.5 ng/ml and 8.5 ng/ml for codeine, and 5.2 ng/ml and15.1 ng/ml for morphine, respectively. For unhydrolysed urine, the LOD and LOQ were 3.0 ng/ml and10.1 ng/ml for codeine, 2.7 ng/ml and 8.1 ng/ml for morphine, 0.8 ng/ml and 1.5 ng/ml for 6-monoacetylmorphine, respectively. In blood, the LOD and LOQ were 0.44 ng/ml and 1.46 ng/ml for codeine, 0.29 ng/mland 0.98 ng/ml for morphine, 0.15 ng/ml and 0.51 ng/ml for 6-monoacetyl morphine, respectively.The validated methods have been applied to 50 urine samples and 40 blood samples (both positive andnegative) and they can be used in routine analyses.
Simultaneus determination of morfine, codeine and 6-acetylmorphine in human urine and blood samples using direct acqueous derivatisation: Validation and application to real cases
CHERICONI, SILVIO;STEFANELLI, FABIO;GIUSIANI, MARIO
2014-01-01
Abstract
tOpiates play a relevant role in forensic toxicology and their assay in urine or blood is usually performed forexample in workplace drug-testing or toxicological investigation of drug impaired driving. The presentwork describes two new methods for detecting morphine, codeine and 6-monoacethyl morphine inhuman urine or blood using a single step derivatisation in aqueous phase. Propyl chloroformate is used asthe dramatizing agent followed by liquid–liquid extraction and gas-chromatography–mass spectroscopyto detect the derivatives. The methods have been validated both for hydrolysed and unhydrolysed urine.For hydrolysed urine, the LOD and LOQ were 2.5 ng/ml and 8.5 ng/ml for codeine, and 5.2 ng/ml and15.1 ng/ml for morphine, respectively. For unhydrolysed urine, the LOD and LOQ were 3.0 ng/ml and10.1 ng/ml for codeine, 2.7 ng/ml and 8.1 ng/ml for morphine, 0.8 ng/ml and 1.5 ng/ml for 6-monoacetylmorphine, respectively. In blood, the LOD and LOQ were 0.44 ng/ml and 1.46 ng/ml for codeine, 0.29 ng/mland 0.98 ng/ml for morphine, 0.15 ng/ml and 0.51 ng/ml for 6-monoacetyl morphine, respectively.The validated methods have been applied to 50 urine samples and 40 blood samples (both positive andnegative) and they can be used in routine analyses.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
