tOpiates play a relevant role in forensic toxicology and their assay in urine or blood is usually performed forexample in workplace drug-testing or toxicological investigation of drug impaired driving. The presentwork describes two new methods for detecting morphine, codeine and 6-monoacethyl morphine inhuman urine or blood using a single step derivatisation in aqueous phase. Propyl chloroformate is used asthe dramatizing agent followed by liquid–liquid extraction and gas-chromatography–mass spectroscopyto detect the derivatives. The methods have been validated both for hydrolysed and unhydrolysed urine.For hydrolysed urine, the LOD and LOQ were 2.5 ng/ml and 8.5 ng/ml for codeine, and 5.2 ng/ml and15.1 ng/ml for morphine, respectively. For unhydrolysed urine, the LOD and LOQ were 3.0 ng/ml and10.1 ng/ml for codeine, 2.7 ng/ml and 8.1 ng/ml for morphine, 0.8 ng/ml and 1.5 ng/ml for 6-monoacetylmorphine, respectively. In blood, the LOD and LOQ were 0.44 ng/ml and 1.46 ng/ml for codeine, 0.29 ng/mland 0.98 ng/ml for morphine, 0.15 ng/ml and 0.51 ng/ml for 6-monoacetyl morphine, respectively.The validated methods have been applied to 50 urine samples and 40 blood samples (both positive andnegative) and they can be used in routine analyses.
|Autori interni:||CHERICONI, SILVIO|
|Autori:||S. Chericoni; F. Stefanelli; V. Iannella; M.Giusiani|
|Titolo:||Simultaneus determination of morfine, codeine and 6-acetylmorphine in human urine and blood samples using direct acqueous derivatisation: Validation and application to real cases|
|Anno del prodotto:||2014|
|Appare nelle tipologie:||1.1 Articolo in rivista|