Neutralizing antibodies are necessary and sufficient for protection against infection with vesicular stomatitis virus(VSV). The in vitro neutralization capacities and in vivo protective capacities of a panel of immunoglobulin G monoclonal antibodies to the glycoprotein of VSV were evaluated. In vitro, neutralizing activity correlated with avidity and with neutralization rate constant, a measure of on-rate. However, in vivo, protection was independent of immunoglobulin subclass, avidity, neutralization rate constant, and in vitro neutralizing activity; above a minimal avidity threshold, protection depended simply on a minimum serum concentration. These two biologically defined thresholds of antibody specificity offer hope for the development of adoptive therapy with neutralizing antibodies.
|Autori:||Bachmann MF; Kalinke U; Althage A; Freer G; Burkhart C; Roost HP; Aguet M; Hengartner H; Zinkernagel RM|
|Titolo:||The role of antibody concentration and avidity in antiviral protection|
|Anno del prodotto:||1997|
|Digital Object Identifier (DOI):||10.1126/science.276.5321.2024|
|Appare nelle tipologie:||1.1 Articolo in rivista|