The present results show that the addition of monodansylcadaverine to rabbit neutrophils stimulates the incorporation of both [3H]inositol and [3H]glycerol into phosphatidylinositol. Monodansylcadaverine also induces the accumulation of phosphatidylinositol 4-phosphate, phosphatidic acid and diacylglycerol. These effects are all dose-dependent and occur at concentrations of monodansylcadaverine which are known to inhibit receptor-mediated endocytosis. Monodansylcadaverine has not effect on phosphatidylinositol hydrolysis or arachidonic acid release, indicating that it acts by increasing de novo synthesis of phosphatidylinositol but not its turnover. It is proposed that the effects of monodansylcadaverine on receptor-mediated endocytosis are mediated, at least in part, by its perturbing effect on lipid metabolism.
|Autori:||Garcia Gil M; van Lookeren-Campagne M.M.; Navarro F.; Esbrit P.; Mato J.M|
|Titolo:||The effect of monodansylcadaverine on the synthesis of phosphatidylinositol by rabbit neutrophils|
|Anno del prodotto:||1984|
|Appare nelle tipologie:||1.1 Articolo in rivista|