Abstract The aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the prolactin (PRL) and PRL receptor's expression in the uterus of mice. For this purpose, 49 Swiss mice were divided into the following groups: GrSS (non-ovariectomized mice given vehicle); GrMET (non-ovariectomized mice treated with metoclopramide); OvSS (ovariectomized mice given vehicle); OvMET (ovariectomized mice treated with metoclopramide); OvMET+17??E (ovariectomized mice treated with metoclopramide and 17?? estradiol); OvMET+MP (ovariectomized mice treated with metoclopramide and micronized progesterone); OvMET+17??E+MP (ovariectomized mice treated with metoclopramide and a solution of 17?? estradiol and micronized progesterone). Immunohistochemical analyzes were evaluated semi-quantitatively. Our results showed that GrMET, OvMET+MP, and OvMET+17??E+MP presented strong PRL expression. OvMET and OvMET+17??E presented mild reaction, while GrSS and OvSS presented weak reaction. Concerning PRL receptor, OvMET+MP and OvMET+17??E+MP showed strong reaction; GrMET, OvSS, and OvMET+17??E showed mild reaction; and GrSS and OvMET showed weak reaction. These findings suggest that progesterone alone or in combination with estrogen may increase the expression of uterine PRL and PRL receptor.
|Autori:||V. C., Do; K. C., Carvalho; G. A., Rosa; Simoncini, Tommaso; P. L., Da; R. R., Marcondes; J. M., Soares; E. C., Baracat|
|Titolo:||The progesterone and estrogen modify the uterine prolactin and prolactin receptor expression of hyperprolactinemic mice.|
|Anno del prodotto:||2015|
|Digital Object Identifier (DOI):||10.3109/09513590.2014.967204|
|Appare nelle tipologie:||1.1 Articolo in rivista|