A series of stereochemically and structurally diverse fluorogenic and chromogenic substrates for hydrolytic enzymes has been synthesized and used to characterize enzyme activity profiles of esterases, lipases, proteases, pepti- dases, phosphatases, and epoxide hydro- lases. The substrates used are particu- larly resilient to nonspecific reactions due to their mechanism of activation.The activities recorded with the individ- ual substrates are therefore remarkably reproducible, and enable us to use the overall pattern of activity as a specific fingerprint for the enzyme sample. Fin- gerprints of activity, and enantio- and stereoselectivity are displayed as arrays of color-scale squares that are easily analyzed visually. Such fingerprints might be useful for quality control, enzyme discovery, and possibly for addressing the issue of functional conver- gence in enzymes.
|Autori:||WAHLER D.; BADALASSI F.; CROTTI P; REYMOND J.-L.|
|Titolo:||Enzyme fingerprints of activity and stereo- and enantioselectivity from fluorogenic and chromogenic substrate arrays|
|Anno del prodotto:||2002|
|Digital Object Identifier (DOI):||10.1002/1521-3765(20020715)8:14<3211::AID-CHEM3211>3.0.CO;2-G|
|Appare nelle tipologie:||1.1 Articolo in rivista|