Three hundred-twenty five ovine droppings from 17 breeding stations in Tuscany (Italy) were examined to detect the presence of yeast. All fecal samples were drawn directly from the rectal ampulla of the animals and submitted to culture examination. One hundred thirty-two of 325 specimens (41%) scored positive, and 141 species were identified. Twenty-three isolates of Candida albicans, 18 of Rhodotorula glutinis, 17 of Cryptococcus humicolus, 16 of C. famata, 14 of C. kefyr, 12 of C. krusei, 8 of C. pelliculosa, 5 of C. guilliermondii, 4 of C. globosa, 4 of Rhodotorula rubra, 3 of C. parapsilosis, 3 of Saccharomyces cerevisiae, 2 of C. intermedia, 2 of C. tropicalis, 2 of C. lambica, 2 of Stephanoascus ciferrii, 2 of Zygosaccharomyces spp., 1 of C. lusitaniae, 1 of C. pulcherrima, 1 of C. laurentii and 1 of Pichia ohmeri were obtained. There was no evidence of C. neoformans. Fungi were recovered in 15 of 17 examined breeding stations with prevalences ranging from 0% to 92% (mean 41.2; sigma = 27.3). The results of this survey could suggest a role of sheep as carriers and reservoirs of potential zoonotic yeasts.