The infrageneric taxonomic structure of Vicia has been re-organised many times, then being 20 major classifications of the genus since Linnaeus. Maxted et al. (1991) divided, by a cytotaxonomic study, the genus Vicia in two subgenera namely Vicia and Vicilla with l7 and 9 sections respectively. The speciation in this genus is accompanied by both chromosome size and large scale variation in nuclear DNA, as well as changes in the frequency of repetitive DNA sequences. In previous paper (Venora et al. 2000) biochemical, cytophotometric and karyological data of cultivated V. faba and of its closest wild relatives of the group Narbonensis have been reported. Isoenzyme analysis in Vicia subgenus vicia has evidenced that V. michauxii, belonging to section Peregrinae (Maxted 1994), is more linked to V. faba than its morphologically closest wild relatives of the Narbonensis complex. Moreover RFLP and RAPD analysis has also suggested closer affinity of V. faba to the section Peregrinae than to the Narbonensis group (Van der Ven et al. 1993). Therefore her we report new evidence on karyotype, chromosome banding, Feulgen DNA determination and analysis of sequences of rDNA spacers in order to enable further clarification of the phylogenetic relationships within Vicia species.
Chromatin organisation and DNA analysis in Vicia species of Peregrinae section.
RUFFINI CASTIGLIONE, MONICA;
2002-01-01
Abstract
The infrageneric taxonomic structure of Vicia has been re-organised many times, then being 20 major classifications of the genus since Linnaeus. Maxted et al. (1991) divided, by a cytotaxonomic study, the genus Vicia in two subgenera namely Vicia and Vicilla with l7 and 9 sections respectively. The speciation in this genus is accompanied by both chromosome size and large scale variation in nuclear DNA, as well as changes in the frequency of repetitive DNA sequences. In previous paper (Venora et al. 2000) biochemical, cytophotometric and karyological data of cultivated V. faba and of its closest wild relatives of the group Narbonensis have been reported. Isoenzyme analysis in Vicia subgenus vicia has evidenced that V. michauxii, belonging to section Peregrinae (Maxted 1994), is more linked to V. faba than its morphologically closest wild relatives of the Narbonensis complex. Moreover RFLP and RAPD analysis has also suggested closer affinity of V. faba to the section Peregrinae than to the Narbonensis group (Van der Ven et al. 1993). Therefore her we report new evidence on karyotype, chromosome banding, Feulgen DNA determination and analysis of sequences of rDNA spacers in order to enable further clarification of the phylogenetic relationships within Vicia species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
