In some previous studies, it has been demonstrated that the final composition of the essential oils can be influenced by the distillation time and some biological activities increased according to the proportion of some types of compounds such as monoterpene alcohols and phenolic derivatives contained in the tested essential oil fractions (1). The aim of the present study was particularly the identification of the bioactive principles of the essential oils from the aerial parts and the roots of Crithmum maritimuL. (2) during their fractional extraction by hydrodistillation. The antioxidant, antibacterial and cytotoxic activities of the essential oils and their collected fractions were also studied. Our results showed that the main constituent of the oils was dillapiole, detected at increasing percentages (67.7-100%) in all the collected fractions during the extraction process. The antioxidant features of all the isolated fractions (F1-F9) of the two oils were also evaluated using DPPH, ABTS, reducing power and paraoxonase assays. The results showed that the IC50 (DPPH) of F9 (aerial parts and roots) were 0.042 ± 0.001 mg/mL and 0.038 ± 0.001 mg/mL, respectively. Moreover, the oil roots exhibited strong growth suppression particularly against Staphylococcus aureus and Enterococcus faecalis. The fraction F9, the aerial parts and the roots essential oils were also tested for their cytotoxic activity and interesting results were noted.
Fractional hydrodistillation and biological evaluation of essential oils from Crithmum maritimum L.
FLAMINI, GUIDO;
2014-01-01
Abstract
In some previous studies, it has been demonstrated that the final composition of the essential oils can be influenced by the distillation time and some biological activities increased according to the proportion of some types of compounds such as monoterpene alcohols and phenolic derivatives contained in the tested essential oil fractions (1). The aim of the present study was particularly the identification of the bioactive principles of the essential oils from the aerial parts and the roots of Crithmum maritimuL. (2) during their fractional extraction by hydrodistillation. The antioxidant, antibacterial and cytotoxic activities of the essential oils and their collected fractions were also studied. Our results showed that the main constituent of the oils was dillapiole, detected at increasing percentages (67.7-100%) in all the collected fractions during the extraction process. The antioxidant features of all the isolated fractions (F1-F9) of the two oils were also evaluated using DPPH, ABTS, reducing power and paraoxonase assays. The results showed that the IC50 (DPPH) of F9 (aerial parts and roots) were 0.042 ± 0.001 mg/mL and 0.038 ± 0.001 mg/mL, respectively. Moreover, the oil roots exhibited strong growth suppression particularly against Staphylococcus aureus and Enterococcus faecalis. The fraction F9, the aerial parts and the roots essential oils were also tested for their cytotoxic activity and interesting results were noted.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
